Middle reaches of Heihe river was chosen as the study area, and meanwhile the core ecological source was identified by biodiversity service, landscape connectivity and habitat quality, and the ecological resistance surface was built by recessive ecological resistance, comprehensive ecological resistance, topography data and street data. Based on the Minimum Cumulative Resistance Model, the ecological corridor network and ecological nodes were identified and a landscape function regionalization was proposed, finally the landscape pattern was optimized from point, line and surface three levels. The results are as follows. 1) The patches with better habitat quality accounted for only 13.77% of the total area, are mainly composed of wetland, high and medium covered grassland and cultivated land, concentrated distributed in the big ups and downs mountains in southeast and the both sides of the main stream of Heihe River. 2) The main ecological corridor throughout the study area from southeast to northwest, the core ecological source and the main ecological corridors are connected by the subsidiary ecological corridors, adjacent main corridors (or sources) are connected by branch corridors, which is mainly distributed in the east ecological nodes are mainly distributed in the weak parts of ecological corridors. 3) Based on the frequency and spatial distribution feature of the minimum cumulative resistance, the area is divided into ecological buffer area, ecological connected area, ecological transition area and ecological edge area four functional areas, construction strategies aiming at ecological sources, ecological corridors and ecological nodes are proposed. The results can provide feasible scientific guidance for ecosystem management in middle reaches of Heihe River.